taqman gene expression Search Results


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Gene expression <t>(CCR5,</t> CCL3, CCL4, CCL5) in the nucleus accumbens of rats injected with cocaine (10 mg/kg) or saline for 4 days was quantified by rt-PCR. Data are presented as the fold- change in mRNA expression compared with saline-injected controls. *p < 0.05, Student’s t-test.
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Side population in normal cartilage. (a) FACS image of the gated side population (SP) and non-SP (NSP) cells isolated via cell sorting. (b) Expression level of <t>ABCG2</t> in SP and NSP cells. The three-fold higher expression of ABCG2 indicates successful isolation of the cartilage SP.
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Side population in normal cartilage. (a) FACS image of the gated side population (SP) and non-SP (NSP) cells isolated via cell sorting. (b) Expression level of <t>ABCG2</t> in SP and NSP cells. The three-fold higher expression of ABCG2 indicates successful isolation of the cartilage SP.
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Side population in normal cartilage. (a) FACS image of the gated side population (SP) and non-SP (NSP) cells isolated via cell sorting. (b) Expression level of <t>ABCG2</t> in SP and NSP cells. The three-fold higher expression of ABCG2 indicates successful isolation of the cartilage SP.
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Side population in normal cartilage. (a) FACS image of the gated side population (SP) and non-SP (NSP) cells isolated via cell sorting. (b) Expression level of <t>ABCG2</t> in SP and NSP cells. The three-fold higher expression of ABCG2 indicates successful isolation of the cartilage SP.
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taqman gene expression assay against tbp hs004271621 m1  (Thermo Fisher)
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Side population in normal cartilage. (a) FACS image of the gated side population (SP) and non-SP (NSP) cells isolated via cell sorting. (b) Expression level of <t>ABCG2</t> in SP and NSP cells. The three-fold higher expression of ABCG2 indicates successful isolation of the cartilage SP.
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Image Search Results


Gene expression (CCR5, CCL3, CCL4, CCL5) in the nucleus accumbens of rats injected with cocaine (10 mg/kg) or saline for 4 days was quantified by rt-PCR. Data are presented as the fold- change in mRNA expression compared with saline-injected controls. *p < 0.05, Student’s t-test.

Journal: Brain, behavior, and immunity

Article Title: Chemokine CCR5 and cocaine interactions in the brain: cocaine enhances mesolimbic CCR5 mRNA levels and produces place preference and locomotor activation that are reduced by a CCR5 antagonist

doi: 10.1016/j.bbi.2019.09.017

Figure Lengend Snippet: Gene expression (CCR5, CCL3, CCL4, CCL5) in the nucleus accumbens of rats injected with cocaine (10 mg/kg) or saline for 4 days was quantified by rt-PCR. Data are presented as the fold- change in mRNA expression compared with saline-injected controls. *p < 0.05, Student’s t-test.

Article Snippet: Quantitative real-time PCR was carried out with TaqMan Fast Advanced Master Mix and the TaqMan Gene Expression Assays for CCR5 (Rn023132969_s1), CCL5 (Rn00579590_m1), CCL4 (Rn00671924_m1), CCL3 (Rn01464736_g1) and the internal control gene 45S rRNA (Rn03928990_g1)/18S rRNA (Hs99999901_s1) using the StepOnePlus Real-Time PCR System (Applied Biosystems).

Techniques: Expressing, Injection, Reverse Transcription Polymerase Chain Reaction

Side population in normal cartilage. (a) FACS image of the gated side population (SP) and non-SP (NSP) cells isolated via cell sorting. (b) Expression level of ABCG2 in SP and NSP cells. The three-fold higher expression of ABCG2 indicates successful isolation of the cartilage SP.

Journal: Arthritis Research & Therapy

Article Title: Mesenchymal progenitor cell markers in human articular cartilage: normal distribution and changes in osteoarthritis

doi: 10.1186/ar2719

Figure Lengend Snippet: Side population in normal cartilage. (a) FACS image of the gated side population (SP) and non-SP (NSP) cells isolated via cell sorting. (b) Expression level of ABCG2 in SP and NSP cells. The three-fold higher expression of ABCG2 indicates successful isolation of the cartilage SP.

Article Snippet: Quantitative real-time RT-PCR (qPCR) was performed using TaqMan Gene Expression Assay probe for ABCG2 (Hs00194979_m1), Sox9 (Hs00165814_m1), Col2a1 IIA (Hs00156568_m1), Col2a1 IIB (Hs01064869_m1), Aggecan (Hs00202971_m1), Col1a1 (Hs00164004_m1), Col10a1 (Hs00166657_m1), Runx2 (Hs00298328_s1), Osterix (Hs00541729_m1), Osteocalcin (Hs01587814_g1), Adiponectin (Hs02564413_S1), and GAPDH (Hs99999905_m1) (All Applied Biosystems, Foster City, CA, USA).

Techniques: Isolation, FACS, Expressing